120th General Meeting of the KCS

Type Poster Presentation
Area Environmental Energy
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code ENVR.P-526
Subject Aerobic Carbon Monoxide Dehydrogenase Immobilized on Electrode for Dissolved Carbon Monoxide Concentration Monitoring
Authors STACY REGINALD*, Yoo Seok Lee1, Hyeryeong Lee1
SCHOOL OF EARTH SCIENCE AND ENVIRONMENTAL ENGINEER, GRADUATE STUDENT GWANGJU INSTITUTE OF SCIENCE AND , Malaysia
1Division of Environmental Engineering, Gwangju Institute of Science and Technology, Korea
Abstract Syngas fermentation is a promising technology for producing bioethanol from lignocellulosic biomass. However, this technology faces several challenges, with substrate inhibition and gas-to-liquid mass transfer limitation due to low solubility of syngas components particularly carbon monoxide in the fermentation broth represent the major bottlenecks. The optimal dissolved carbon monoxide concentration in fermentation process is crucial because several microorganisms such as ‘acetogens’ that grow chemoautotrophically on carbon monoxide are inhibited by high dissolved carbon monoxide concentration. The aim of this study is to develop an enzyme-based biosensor for dissolved carbon monoxide monitoring which is essential for enhancing gas-to-liquid mass transfer and overcome substrate inhibition in syngas fermentation process. Carbon monoxide dehydrogenase-based biosensor is proposed for determination of dissolved carbon monoxide concentration. Carbon monoxide dehydrogenase (CODH) from the aerobic carbon monoxide utilizing carboxidotrophic eubacterium, Hydrogenophaga pseudoflava, unlike CODH from other aerobic carboxidobacteria produce catalytically fully competent CODH under heterotrophic condition. CODH from Hydrogenophaga pseudoflava is immobilized on electrode which was firstly proposed to monitor dissolved carbon monoxide. The electrochemical behaviors and electrocatalytic performance of this enzyme-based biosensor towards dissolved carbon monoxide could be used to monitor dissolved carbon monoxide concentration that will be vital for understanding, predicting and optimizing syngas fermentation process.
E-mail stacyreginald@gist.ac.kr