120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room C311+C312
Time THU 10:07-:
Code ANAL1.O-24
Subject Effect of high fat diet on mouse brain lipidomes by nUPLC-ESI-MS/MS : Cortex, Hippocampus, Hypothalamus, & Olfactory bulb
Authors JongCheol Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Lipids are known to be involved in various neuronal functions in brain, which is one of the most important and complex organ in the central nervous system. As brain plays a number of roles including cognition, memory, learning, and metabolic control, brain dysfunction can eventually lead to a variety of diseases. Recently, lipidomic analysis in relation to brain diseases has attracted much attention. High fat diet (HFD) can cause insulin resistance in the brain tissues by inducing obesity through diet. Although the effects of HFD on brain health have been studied in pathology, the relationship between HFD and brain lipid profiles has not been extensively studied. In this study, four different brain tissues (cortex, hippocampus, hypothalamus, and olfactory bulb) from mouse upon high fat diet programs have been analyzed using nanoflow ultrahigh pressure LC-ESI-MS/MS (nUPLC-ESI-MS/MS) for the investigation of HFD effect on the changes in the lipid profiles. Mice were fed with the various dietary plans: 8 weeks normal control (N), and weight gain (F), 16 weeks normal control (NNN), weight maintenance (FNN), weight gatin (NNF), and weight cycling (FNF), As a result, 270 lipids including phospholipid, glycerolipid, and sphingolipid were quantified and statistical analysis was applied.
E-mail jchul@yonsei.ac.kr