120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room C311+C312
Time THU 10:09-:
Code ANAL1.O-25
Subject Analysis of HDL from coronary artery disease patients through bottom-up and top-down proteomic approach using flow field-flow fractionation and mass spectrometry
Authors Jae-Hyun Lee, JoonSeon Yang, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Coronary artery diseases (CAD) is a disease caused by narrowing of the coronary arteries of the heart by plaque, fat or other substances. Narrow coronary artery interfere with blood flow to heart and may even cause damage in heart or eventually lead to death. High-density lipoprotein (HDL) has been reported to be associated with CAD as marker molecules. Lipoprotein is known as a macromolecules that transports lipids through the blood. These lipoproteins are divided into different classes depending on their density and specific role. HDL is composed of lipid and various proteins including apolipoproteins. One of the roles of HDL to transport excess cholesterol to the liver and regulate cholesterol levels in cells, blood vessels, and blood. Transportation of cholesterol by HDL helps to remove excessive cholesterol out of cells, blood, and blood vessel wall. Recent studies have been reported that apolipoprotein A-I in HDL goes through oxidation in severity of CAD. Flow field-flow fractionation (FlFFF) is widely used to separate macromolecules and nanoparticles according to their hydrodynamic diameter. In this study, HDL from human plasma of CAD patients was separated by FlFFF. Miniaturized asymmetrical FlFFF (AF4) channel was coupled on-line to electrospray ionization mass spectrometry (ESI-MS) for a high speed separation of lipoproteins and top-down proteomic analysis for various proteins including ApoA-1 from HDL were analyzed in order to investigate the change in HDL-related proteins caused by severity of CAD.
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