120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room C311+C312
Time THU 10:11-:
Code ANAL1.O-26
Subject Steric Transition Phenomena upon Field Decay Patterns Using Frit-inlet Asymmetrical Flow Field-Flow Fractionation
Authors Young Beom Kim*, Lee Hye Jin, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Flow field-flow fractionation (FlFFF) is a separation technique that can separate macromolecules by size without stationary phase. The separation mode of FlFFF can be divided into normal and steric/hyperlayer mode; particles with sizes smaller than 1µm are operated in normal mode while those larger 1µm are operated in steric/hyperlayer mode. During the focusing/relaxation process, smaller particles diffuse more and equilibrate at a position higher from the channel than larger particles. As a result, The smaller particles are located in faster flow stream and eluted earlier than the larger particles. In the steric/hyperlayer mode, where the particle size is larger than 1µm, Brownian diffusion becomes negligible and particles are elevated to a certain height by lift force. Since the larger particles have higher lift forces than the smaller particles, they elute earlier. Steric transition is a phenomenon that particle elutes by a combination of normal mode and steric mode in FlFFF. Frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) operates by stopless flow without focusing process. In this study, steric transition phenomenon is systematically monitored with polystyrene particles by FI-AF4 coupled with UV/visible detector and multi-angle light scattering. Depending on the strength and gradient of the field, the particle size at which the steric transition occurs varies. Therefore, it is possible to increase the range of particle size separated in the normal mode.
E-mail bum5203@yonsei.ac.kr