120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room C311+C312
Time THU 10:13-:
Code ANAL1.O-27
Subject Profiling of lipoproteins from patients with mild cognition impairment and Alzheimer’s disease by asymmetrical flow field-flow fractionation and nUPLC-ESI-MS/MS
Authors SAN HA KIM, JoonSeon Yang, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Recently, as the average life span of people is increasing, the number of elderly population has been rising sharply and cognitive impairment disorders with aging factor such as Alzheimer’s disease have become common. Amyloid-beta plaques is known to be a major cause of Alzheimer’s disease and kills neighboring brain cells, which leads to dysfunction in cognitive abilities. Symptoms of Alzheimer’s disease’s are usually developed decades after the accumulation of amyloid-beta. Therefore, the rate of disease progression is even faster when symptoms start to appear. About 10 percent of patients with mild cognitive imparirment, a pre-stage of Alzheimer’s disease, end up diagnosed with Alzheimer’s disease within a year. Several studies have reported that high levels of low-density lipoprotein(LDL) and low levels of high-density lipoprotein(HDL) is affected amyloid-beta. Therefore, it is important to understand lipid metabolism from separating HDL and LDL, separately. In this study, lipids from human plasma samples of healthy controls, patients with mild cognition impairment, and Alzheimer’s disease patients were analyzed. Lipoproteins were fractionated by asymmetrical flow-field flow fractionation and the lipids were extracted from the collected fractions of HDL and LDL and structurally identified from collision-induced dissociation of nanoflow ultrahigh-pressure liquid chromatography-electrospray ionization-tandem mass spectrometry (nUPLC-ESI-MS/MS). More than 300 lipids were identified. Identified lipids are quantified from individual samples to evaluate the difference between the two groups in comparison to those of healthy controls.
E-mail sanhakim92@yonsei.ac.kr