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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists I |
| Room No. |
Room C311+C312 |
| Time |
THU 10:45-: |
| Code |
ANAL1.O-43 |
| Subject |
Determination of Fenpyroximate from Honey by LC-MS/MS |
| Authors |
JinMun Kim, JUN SEOK KIM1, Hyun-Woo Cho2, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Korea Polytechnics, Korea
2Department of Natural Science Chemistry, Kyonggi University, Korea
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| Abstract |
Fenpyroximate is one of the insecticides used in beekeeping farms. This may remain in honey, which is regulated and banned in some countries. Honey have a complex matrix and therefore, it proceeded by combining Liquid-Liquid Extraction (LLE) and Solid Phase Extraction (SPE) for clean-up and concentration. The parameters for clean-up and concentration of the sample were optimized and validated. The parameters such as pH of sample, type of SPE cartridge, volume of extraction solvent, sample volume, volume of elution solvent, type of elution solvent were evaluated. were optimized. The chromatographic separation by LC-MS/MS system was achieved on an InfinityLab Poroshell 120 EC-C18 (3.0 mm i.d. × 50 mm length, 2.7 μm particle size) column using isocratic elution with water and acetonitrile (30:70). Ionization of the analyte was done by electrospray ionization (ESI) and quantitation was performed by multiple reaction monitoring (MRM). The limit of detection (LOD) and limit of quantitation (LOQ) from the spiked sample were 1.56 and 5.00 μg/kg respectively. The accuracy and precision in the working range (5.00-50.0 μg/kg) were 87.99-119.06 % and 0.68-14.29 % (RSD), respectively. The correlation coefficient (r2) for the linear equations was obtained in the range of 0.9914-0.9977. The established method was applied to monitor thirty types of commercially available honey. |
| E-mail |
jm12355@naver.com |
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120th General Meeting of the KCS