120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room C308+C309
Time FRI 09:34-:
Code ANAL2.O-10
Subject Near-infrared fluorescent probes for the detection of alkaline phosphatase activity in-vivo imaging
Authors Chul Soon Park, Tai Hwan Ha, KyungKwan Lee1, Chang-Soo Lee2,*
Center for Bio Monitoring Research, Korea Research Institute of Bioscience & Biotechnology, Korea
1Center for Bio Nano Research, Korea Research Institute of Bioscience & Biotechno, Korea
2Center for Bio Nano Research, Korea Research Institute of Bioscience & Biotechnology, Korea
Abstract Alkaline phosphatase (ALP) is a crucial biological enzyme for osteoblast activity during early osteoblast differentiation, but only few biologically compatible methods are available for its detection. Here, we developed highly selective, sensitive and fast responsive new near-infrared (NIR) fluorescence probes, NIR-Phos 1 and NIR-Phos 2, for the fluorescent detection of ALP. The NIR probes also displayed desired properties such as high stability and selectivity, showing a highly rapid “turn-on” fluorescence response rate completed within 1.5 min, on adding 0.1 U/mL ALP. By taking advantage of the superior properties of the NIR probes, we have demonstrated its utility in real-time monitoring of both the concentration- and time-dependent variations of endogenous ALP in living cells and animals, thereby providing a potentially powerful approach for probing ALP activity in biological systems.
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