|
Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room C308+C309 |
Time |
FRI 09:34-: |
Code |
ANAL2.O-10 |
Subject |
Near-infrared fluorescent probes for the detection of alkaline phosphatase activity in-vivo imaging |
Authors |
Chul Soon Park, Tai Hwan Ha, KyungKwan Lee1, Chang-Soo Lee2,* Center for Bio Monitoring Research, Korea Research Institute of Bioscience & Biotechnology, Korea 1Center for Bio Nano Research, Korea Research Institute of Bioscience & Biotechno, Korea 2Center for Bio Nano Research, Korea Research Institute of Bioscience & Biotechnology, Korea |
Abstract |
Alkaline phosphatase (ALP) is a crucial biological enzyme for osteoblast activity during early osteoblast differentiation, but only few biologically compatible methods are available for its detection. Here, we developed highly selective, sensitive and fast responsive new near-infrared (NIR) fluorescence probes, NIR-Phos 1 and NIR-Phos 2, for the fluorescent detection of ALP. The NIR probes also displayed desired properties such as high stability and selectivity, showing a highly rapid “turn-on” fluorescence response rate completed within 1.5 min, on adding 0.1 U/mL ALP. By taking advantage of the superior properties of the NIR probes, we have demonstrated its utility in real-time monitoring of both the concentration- and time-dependent variations of endogenous ALP in living cells and animals, thereby providing a potentially powerful approach for probing ALP activity in biological systems. |
E-mail |
log7194@naver.com |
|