120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room C308+C309
Time FRI 09:38-:
Code ANAL2.O-12
Subject Contemporary Multispectral Three Dimensional Observation of Intracellular Organelles via Enhanced Dark-field Super-resolution Microscopy
Authors Suresh Kumar Chakkarapani, Seungah Lee1, Soyeong Ju, Seong Ho Kang1,*
Department of Chemistry, Kyung Hee University, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
Abstract An innovative fluorescence-free super-localization approach for imaging intracellular organelles contacts at three dimension using dual-wavelength enhanced dark field microscopy (EDF). Mitochondria associated endoplasmic reticulum [mito-ER] was imaged by labelling the organelles with plasmon nanoparticles (NPs) of different scattering wavelength. The dual-wavelength synchronized EDF setup allows a concurrent imaging of both organelles under physiological conditions. The three dimensional super-localization of the NPs was achieved by fitting the center coordinates by least-cubic algorithm. The reconstructed super-resolution images resolved controversy over the distance between the intracellular organelles at functional contacts. The distance between the organelles was measured to be 45 nm, the distance fits to be the true feature of the organelles at functional contact site. The results designate to be the novel and reliable approach for the analysis of intracellular organelles at three dimension for accurate information under physiological conditions.
E-mail sureshsrkmv@gmail.com