120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room C308+C309
Time FRI 09:46-:
Code ANAL2.O-16
Subject Ultra-sensitive Immunodetection of Cancer Antigen 125 based on Enhanced Plasmonic Scattering of Nano Probe by Dual-mode Wavelength-dependent Enhanced Dark-field Super-resolution Microscopy
Authors Soyeong Ju, Seungah Lee1, Suresh Kumar Chakkarapani, Seong Ho Kang1,*
Department of Chemistry, Kyung Hee University, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
Abstract A ultra-sensitive immunodetection was achieved for the biomarker cancer antigen 125 (CA125), which was developed based on selected enhanced detection immunotag by dual-mode wavelength-dependent enhanced dark-field (EDF) microscopy. For simultaneous dual-detection, an color digital camera and electron multiplying cooled charge-coupled device camera were used for quantitative and qualitative analysis, respectively, based on the dark-field scattering images. To increase the efficacy of the scattering signal;, various size of different plasmon nanoparticle (i.e., gold nanoparticles, 5 nm, 12 nm, 20 nm, 100 nm and 250 nm; silver nanoparticles, 20 nm, 30 nm, 40 nm, 80 nm and 100 nm) were used as the detection tags and investigated with wavelength dependence of the light source, and the quantum efficiency of the electron-multiplying charge-coupled device camera, 40-nm silver nanoparticle (SNP) was selected as an optimum fluorescence-free probe. CA125 was screened at single-molecule level and quantitatively analyized by measuring the scattering signals of 40-nm SNP on gold-nanodots arraychip. CA125 was screened at single-molecule level and quantitatively analyzed with lowest possible possible LOD (4 ㅕU/mL, S/N = 3) and a wide dynamic detection range of 4 ㅕU/mL-80 U/mL (R = 0.9935), which was a over the 100 times lower LOD and wider dynamic range than previous researches. Dual-mode EDF based optical property of the plasmon scattering signal of metal nanoparticles allowed us for a of disease related biomolecules at single-molecule level for early diagnosis of life threatening diseases.
E-mail soyeongju@naver.com