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Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room C308+C309 |
Time |
FRI 10:15-: |
Code |
ANAL2.O-28 |
Subject |
Fast Direct Apolipoprotein E Genotyping for Alzheimer’s by Multi-channel Microchip Electrophoresis |
Authors |
Yucheng Sun, Nain Woo, Seong Ho Kang1,* Department of Chemistry, Kyung Hee University, Korea 1Department of Applied Chemistry, Kyung Hee University, Korea |
Abstract |
A direct apolipoprotein E (apoE) genotyping-based multi-channel microchip electrophoresis (ME) method was developed for fast high-throughput screening of Alzheimer. The target apoE polymorphism genes (i.e., 112 bp, 253 bp, 308 bp, 444 bp, and 514 bp DNA) were amplified by only 10 cycles of one-step multiplex polymerase chain reaction (PCR) from whole blood without DNA extraction. The amplified target DNA fragments were detected in 20 s by a programmed electric field strength (PEFS)-based single-channel ME. According to the optimum conditions of single-channel ME, target DNA fragments were separated using various multi-channel ME with three and five parallel channels, which simultaneously detected within 20 s without any loss of resolving power or reproducibility. The proposed multi-channel ME-PEFS assay technique provides a simple and accurate method for fast high-throughput screening of Alzheimer's disease, which can be simply apply to more large number of parallel channels for more high-throughput screening. |
E-mail |
sun.yc900911@gmail.com |
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