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Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room C308+C309 |
Time |
FRI 10:17-: |
Code |
ANAL2.O-29 |
Subject |
Identification of Diverse Types of Monosaccharide Derivative Isomers By Host-guest Complexation with Cucurbit[7]uril |
Authors |
Hyun Hee L. Lee, Hugh Inkon Kim* Department of Chemistry, Korea University, Korea |
Abstract |
Distinguishing constituent monosaccharides in target carbohydrate using mass spectrometry (MS) remains challenging due to their undistinguishable fragmentation patterns induced by their isomeric structures and similar physical properties. Although a carbohydrate is composed of diverse monosaccharide/monosaccharide derivative species, previous methods focused on one or two types of monosaccharides and monosaccharide derivatives. Therefore, it is necessary to invent a novel approach to be developed as a comprehensive method for identification of diverse types of monosaccharides and monosaccharide derivatives.
Our study reveals that the combination between host-guest and gas-phase chemistry is effective for identifying diverse types of monosaccharide derivative isomers. Four different types of monosaccharide derivative isomers were investigated herein as target monosaccharide derivative species. A host molecule, cucurbit[7]uril (CB[7]), was utilized herein due to its high recognition ability in monosaccharides. It was observed that each monosaccharide derivative forms gas-phase complex with CB[7] while most of bindings between the CB[7] and target guests are negligible in aqueous solution. Tandem MS study shows that the monosaccharide derivatives have different fragmentation patterns in their complexes with CB[7], allowing their identification. Structural studies using ion mobility MS and computational calculations suggest that the slight structural differences between the isomers are augmented by their distinct host-guest interactions with CB[7] in the gas-phase. Overall, the present study reveals that our gas-phase host-guest system can be developed as a comprehensive method for qualitative analysis of constituent monosaccharides in target carbohydrates.
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E-mail |
hyun630@korea.ac.kr |
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