120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room C308+C309
Time FRI 10:23-:
Code ANAL2.O-32
Subject Highly sensitive detection of lethal infectious pathogen using SERS-based lateral flow assay
Authors Rui Wang, Kihyun Kim, JAEBUM CHOO*
Department of Bionano Technology, Hanyang University, Korea
Abstract Effective identification of pathogenic bacteria is important for biodefense. In recent years, microbiological culture and colony counting, polymerase chain reaction (PCR) or immunoassay has been considered as a gold standard for the detection of pathogens including toxin, bacteria and virus. However, these methods cannot meet the requirement of rapid and highly sensitive detection because they need long sample preparation time and have limited detection sensitivity. Therefore, it is still urgent to develop a new method for the rapid and sensitive detection of pathogens for preparedness of bioterrorism. In this presentation, we introduce a new pathogen assay technique using a surface-enhanced Raman scattering (SERS)-based lateral flow assay. Two different antigens, including botulinum toxin A and Yersinia pestis have been selected as detection targets. According to our assay results, the limit of detection of botulinum toxin A and Yersinia pestis using the SERS-based lateral flow platform was 10 pg/mL and 102 CFU/mL, respectively, which are three orders of magnitude more sensitive than that of commercial rapid kit. Thus, the proposed SERS-based lateral flow assay has a strong potential to be a valuable tool in the early detection of a specific pathogen due to its excellent analytical sensitivity.
E-mail wangrui.1985@163.com