120th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room C308+C309
Time FRI 10:25-:
Code ANAL2.O-33
Subject Rapid and sensitive detection of highly risk pathogens using SERS-based lateral flow assay
Authors Kihyun Kim, Rui Wang, JAEBUM CHOO*
Department of Bionano Technology, Hanyang University, Korea
Abstract Bioterrorism has been received great concerns after the 2001 anthrax attacks in United States. Highly risk pathogens used in the bioterror are greatly contagious and also have a high fatality. To minimize life damages from bioterror, rapid and sensitive detection methods of specific pathogen is urgently required. To date, various detection methods including direct counting of bacteria, polymerase chain reaction (PCR), and immunoassay have been developed but they do not satisfy the requirements in the field due to complicated sample pre-treatment procedures, long detection time and low sensitivity. To resolve those problems, we recently developed a SERS-based detection technique for the detection of various pathogens. In this poster, we introduce a new pathogen assay method using a SERS-based lateral flow assay. Two different pathogens, Bacillus anthracis and Francisella tularensis have been selected as detection targets. In the case of Francisella tularensis, the limit of detection measured by the SERS-based lateral flow platform was estimated to be 102 CFU/mL, which is three orders of magnitude more sensitive than that of commercial ELISA kit. Therefore, the proposed SERS-based lateral flow assay technique has a strong potential to be used as a valuable tool in the early detection of a specific pathogen due to its simplicity and excellent analytical sensitivity.
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