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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-256 |
| Subject |
Structure of hetero-peptide oligomers studied with force-based AFM |
| Authors |
Eun Ji Shin, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
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| Abstract |
Alzheimer’s Disease(AD) and Parkinson’s Disease(PD) are neurodegenerative diseases resulting in progressive degeneration or death of neuron cells. These are associated with the aggregation of misfolded proteins ‘Amyloid-beta (Aβ)’ and ‘Alpha-synuclein (α-syn)’ at each of the disease. It is known that Aβ and α-syn ‘oligomer state’, which represent intermediates in the fibril formation process may be primarily responsible for pathogenesis. And growing evidence suggests that Aβ and α-syn might exist in one oligomer and promote the aggregation of one another in AD/PD pathology causing the disease to worsen further. However, studies of Aβ and α-syn mixture oligomer have been sparse and indirect.
Here, oligomer surface information could be characterized at the single-molecule level by AFM (Atomic Force Microscopy) and AFM tip modification process. With the AFM imaging mode and force mapping mode, morphology image of oligomer and surface information can be obtained with each Peptide specific antibody-tethered tip. Through overlapping the image and the force map, we can get information about specific target molecule’s distribution. And with the AFM tip modification process, Dendron-coated surface of the tip has an ample spacing inducing to a single interaction between the tip and oligomer. This research is able to overcome the limitations of traditional ensemble averaging methods and analyze in a new way that they did not.
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| E-mail |
shinej@postech.ac.kr |
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121st General Meeting of the KCS