121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-257
Subject Quantification of Tumor-associated Circulating Free DNA with Atomic Force Microscopy
Authors Woo Cheol Shim, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
Abstract Unlike conventional invasive biopsy method, non-invasive liquid biopsy has come into the spotlight to obtain information about cancer from present biomarkers such as circulating free DNA (cfDNA), exosomes, circulating tumor cells (CTCs) in patient`s blood. Especially, it can be used for diagnosis and prognosis of cancer to detect genetic alterations of circulating free DNA (cfDNA) related to cancer. In the past few years there have been many endeavors to reveal correlation between cfDNA and cancer. Here we propose a new methodology using atomic force microscopy (AFM) to quantify tumor-specific genetic aberrations involved in point mutation and methylation change of cfDNA with very low abundance. Quantification of cfDNA is under way by using MutS protein with the ability to recognize DNA mismatches and 5-methylcytidine-specific antibody. This unprecedented liquid biopsy followed by AFM will be a great help for conquering cancer. Reference 1. Crowley, E.; Di Nicolantonio, F.; Loupakis, F.; Bardelli, A., Liquid biopsy: monitoring cancer-genetics in the blood. Nat. Rev. Clin. Oncol. 2013, 10 (8), 472. 2. Lee, Y.; Kim, Y.; Lee, D.; Roy, D.; Park, J. W., Quantification of Fewer than Ten Copies of a DNA Biomarker without Amplification or Labeling. J. Am. Chem. Soc. 2016, 138 (22), 7075-7081.
E-mail Shimwc@postech.ac.kr