121st General Meeting of the KCS

Type Poster Presentation
Area Electrochemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code ELEC.P-569
Subject Chip-based electrochemical immunoassay free of reference and counter electrodes with 3D interdigitated electrode array
Authors SUNMI LEE, TAEK DONG CHUNG1,*
Program in Nano Science and Technology, Seoul National University, Korea
1Division of Chemistry, Seoul National University, Korea
Abstract We demonstrate that the three-dimensional (3D) interdigitated array (IDA) chip operates as a electrochemical biosensor without reference and counter electrodes. Reference and counter electrodes are considered essential components to apply potential of the magnitude that can trigger the intended redox process but they are unwieldy for chip-based electrochemical detection. Using the 3D IDA electrode configuration, we propose a unique electrochemical system that can control applied potential to the pair of working electrodes even though reference and counter electrodes do not exist on the chip separately. The electrochemical potential of the 2-electrode (2E) system is defined by the potential of the electron transfer mediator, poly(methylene green), which is immobilized with poly(dopamine) on the ITO surface by electropolymerization. The 3D IDA electrode chip in the 2E system sensitively operates as an electrochemical immunosensing platform. Creatine Kinase-MB in human serum was measured down to ~ pg /mL level with the chip. Therefore, the 3D IDA in the 2E system is a simple platform that can achieve electrochemical detection with nL level of sample volume sensitively in miniaturized multiplex immunoassay.
E-mail smlee01@snu.ac.kr