|
|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-258 |
| Subject |
Femtomolar-Range Profiling of MicroRNA Using Metal-Enhanced Fluorescence Nanoparticles
|
| Authors |
Soohyun Park, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
|
| Abstract |
MicroRNAs(miRNAs) are small noncoding single-stranded RNAs(~22nt) that are involved in protein transcription, physiological mechanisms such as development, differentiation, cell proliferation and apoptosis. They are also known to be associated with many diseases such as cancer, Alzheimer’s disease, myocardial disease. miRNA is shorter than general RNA, thus miRNA is amplified by PCR. In particular, the role of miRNAs in cancer has been extensively studied. miRNA analysis can be useful for early diagnosis or treatment of diseases.
Currently, Polymerase Chain Reaction (PCR) is a conventional method to detect miRNA. However, it causes error in amplification process and analysis sensitivity is relatively low. In order to overcome the limitations of the conventional technology, I will improve the sensitivity more than 100 times using metal-enhanced fluorescence nanoparticles and microarray.
Here we suggest new approaches to supersensitive analysis of miRNA using metal-enhanced fluorescence (MEF) nanoparticles and microarray. To detect miRNA, we use S9.6 antibody that is DNA/RNA hybrid-specific mouse monoclonal antibody. This approach is expected to perform parallel analysis with femtomolar-range sensitivity increased several hundred times more than the conventional methods.
Reference
1. Z. Hu, et al. Nucl. Acid Res. 2006, 34, e52
2. B. J. Hong, et al. Nucl. Acid Res. 2005, 33, 12
3. J. E. Park, et al. Nano Lett. 2016, 16, 7962
|
| E-mail |
parksoo@postech.ac.kr |
|
121st General Meeting of the KCS