121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-259
Subject Quantitative Analysis of DISC1 in a Single Cell with Atomic Force Microscopy
Authors Ji-seon Lim, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
Abstract Schizophrenia is a mental disorder characterized by abnormal social behavior and failure to understand what is real. Several studies have shown that unregulated expression or altered protein structure of disrupted in schizophrenia 1 (DISC1) may predispose individuals to the development of schizophrenia, clinical depression, bipolar disorder, and other psychiatric conditions. Therefore, identification of protein level or post-translational modifications of DISC1 can provide targets for diagnosis and treatment of schizophrenia. Especially, post-translational modifications of DISC1 have been studied as a mechanism to regulate the interaction with these proteins under specific conditions. Recently, several studies have been discovered that phosphorylation of DISC1 plays an important role in the process of neural development. Conventional methods for protein analysis including western blotting, ELISA, and immunofluorescence have some problems that they have low detection limit and use fluorescent molecules. Also, we can’t know the information about a single cell because they measure ensemble average of molecules. Therefore, we analyze target proteins which are DISC1 and phospho-DISC1 in a single cell using atomic force microscopy (AFM). AFM have the capability to detect one to one interaction between antigen and antibody. By using nanoarray of fluidic force microscopy (FluidFM), trace amount of proteins can also be analyzed quantitatively without amplification and labeling in a single cell. Through quantitative analysis of proteins in a single cell level, we can establish new single cell technology and also understand cell-to-cell variation.
E-mail jslim@postech.ac.kr