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| Type |
Poster Presentation |
| Area |
Inorganic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
INOR.P-36 |
| Subject |
Structural analyses of zinc finger proteins for specific interaction |
| Authors |
Ha Ra Jang, Seung Jae Lee*
Department of Chemistry and Research Institute for Molecular Biology and Genetics, Chonbuk National University, Korea
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| Abstract |
Zinc finger proteins are among the most extensively applied metalloproteins in the field of biotechnology due to their unique structural and functional aspects as transcriptional and translational regulators. The classical zinc fingers are the largest family of zinc proteins and they provide critical roles in physiological systems from prokaryotes to eukaryotes. Two cysteine and two histidine residues (Cys2His2) coordinate to the zinc ion for the structural functions to generate a ββα fold, and this secondary structure supports specific interactions with their binding partners including DNA, RNA, lipids, proteins, and small molecules. In this presentation, the structural similarity and differences of well-known Cys2His2 type zinc fingers such as zinc interaction factor 268 (ZIF268), transcription factor IIIA (TFIIIA), GAGA, and Ros will be explained. These proteins perform their specific roles in species from archaea to eukaryotes and they show significant structural similarity; however, their aligned amino acids present low sequence homology. These zinc finger proteins have different numbers of domains for their structural roles to maintain biological progress through transcriptional regulations from exogenous stresses. The superimposed structures of these finger domains provide interesting details when these fingers were applied to specific gene binding and editing. The structural information in this study will aid in the selection of unique types of zinc finger applications in vivo and in vitro approaches because biophysical backgrounds including complex structures and binding affinities aid in the protein design area. |
| E-mail |
gkfk0209@jbnu.ac.kr |
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121st General Meeting of the KCS