121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-261
Subject Characterization of Novel Caleosin/Peroxygenase in Rice Oxylipin Synthesis
Authors Kyoungwon Cho, SEREYVATH YOEUN, Oksoo Han1,*
Department of Biotechnology, Chonnam National University, Korea
1Department of Molecular Biotechnology, Chonnam National University, Korea
Abstract The accumulation of transcriptomic data from diverse stress conditions, development stages, organs and cultivars and gene co-expression network analysis can provides precise information for identifying the function of novel genes. Here we report a comparative analysis of rice genome-wide expression patterns to investigate novel genes involved in oxylipin biosynthesis and their functional characteristics. The oxylipin constitutes a family of oxygenated products which are formed from fatty acids and plays curial roles in plant stress and development responses. We gathered 210 transcriptome datasets resulted from Agilent rice 44K DNA chip in NCBI GEO and our research team. Pearson correlation analysis was performed between 34 jasmonic acid (JA, representative oxylipin) biosynthesis genes (17 LOXs, 2 AOSs, 3 HPLs, 1 AOCs, and 11 OPRs) and all genes (43494) on rice DNA chip. We selected genes with high correlation coefficient value (r > 0.7 or < -0.7), annotated their functions and then found genes involved in lipid epoxidation, hydroxylation and omega-hydroxylation, including 32 genes encoding caleosin/peroxygenase (3), cytochrome P450 78A (6), 86A (11), 89A (7) and 94A (5). Co-expression network analysis between 34 JA genes and 32 novel genes showed that there exist at least two oxylipin pathways in rice (HPL and AOC pathways) and they are reversely correlated. Furthermore, to characterize three novel genes encoding caleosin/peroxygenase (OsPXG), the genes (OsPXG7, OsPXG8, OsPXG9) were cloned and their biochemical features have been studied.
E-mail kw.cho253@gmail.com