121st General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists I
Room No. Room 201A
Time THU 09:15-:
Code ANAL1.O-2
Subject Optimization of Purification Methodology for Emerging PET Radiometal Zr-89
Authors Chirag Vyas, JEONG HOON PARK*
Radiation Instrumentation Division, Korea Atomic Energy Research Institute, Korea
Abstract Zr-89 with the favorable nuclear decay kinetics and chemical properties is an appealing radiometal for its application in immuno-PET using radiolabeled monoclonal antibodies. Nevertheless, non-availability of the reliable and robust purification techniques makes the use of Zr-89 limited. Hydroxamate based extraction chromatographic resin has been proved to be idiosyncratic amongst other purification techniques for Zr-89. Nevertheless, detailed optimized purification studies are limited and it will be of great interest to permute and combine different parameters to further develope novel and straightforward purification methodologies. In this study we have made efforts to optimize the purification of Zr-89 in oxalate as well as chloride form using commercially available hydroxamate based extraction chromatographic resin. The comparative comprehensive studies using different parameters viz. amount of resin, acid concentration, loading, washing and elution patterns of the column with the different column operation viz. gravity, vacuum box and syringe pump was carried out. The advanced optimized conditions showed extraordinary purification capabilities over other hydroxamate resin based separation methods available in the literature. Lower HCl (≤ 1.5 M) and oxalic acid (0.05 M) as compared to that with ~2-6 M and 1 M concentration for loading of the dissolved target and elution of final Zr-89 respectively are promising. Moreover, high radionuclidic and radiochemical purity Zr-89 (≥ 99.99 %) can be obtained in 0.05 M of oxalic acid and/or 1 M HCl with ≥ 99 % of separation efficiency in ≤ 1 mL of the final product volume using the optimization studied proving its potentials as a novel methodology for Zr-89 separation and purification.
E-mail vyaschiragk@yahoo.co.in