|
Type |
Poster Presentation |
Area |
Organic Chemistry |
Room No. |
Event Hall |
Time |
4월 19일 (목요일) 11:00~12:30 |
Code |
ORGN.P-358 |
Subject |
A coumarin-naphthalimide hybrid as a ratiometric fluorescent probe for hNQO1 |
Authors |
Sun Young Park, Min Hee Lee* Department of Chemistry, Sookmyung Women's University, Korea |
Abstract |
Human NAD(P)H:quinone oxidoreductase (hNQO1) promotes a reduction of quinones to hydro quinones in the presence of NADH. It is reported that the hNQO1 activity is higher in cancer cells than in normal cells and it is also associated with a drug resistance factor of cancer cells. Here, we presented a coumarin-naphthalimide hybrid (1) containing a trimethyl lockquinone designed as the trigger group as a selective ratiometric fluorescent probe for detecting hNQO1. The trimethyl lockquinone moiety of 1 could be reduced by hNQO1 into hydroquinone which subsequently cyclized, resulting in a production of 2 as well as a ratiometric fluorescence change at 450 and 540 nm. In addition, probe 1 was found to be highly selective for hNQO1 activity over other biologically relevant species, including metals, ROS, and anions. Morevoer, we studied enzyme kinetics of 1 using Michaelis-Menten plot, and enzyme efficiency of 1 turned to be kcat/Km = 2.184 x 104 (± 0.01821) M-1s-1. |
E-mail |
1411437@sookmyung.ac.kr |
|