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|
| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ORGN.P-360 |
| Subject |
Development of hemicyanine-based fluorescent probes for the detection of human NAD(P)H:quinone oxidoreductase (hNQO1) and its application in live human cells |
| Authors |
Jinju Lee, Min Hee Lee*
Department of Chemistry, Sookmyung Women's University, Korea
|
| Abstract |
A new hemicyanine-based fluorescent probes (1 and 2) were developed for detection and imaging of NAD(P)H:quinone oxidoreductase (hNQO1) in human cells. Probes show a broad absorption band at 480 nm and a very weak fluorescent emission at 560 nm. However, upon the addition of hNQO1, a significant fluorescence increase at 560 nm and color change from yellow to red were observed in physiological conditions. In addition, these changes were not observed in the case of other biologically relevant species, including metal ions, redox species, and anions, in similar conditions. Moreover, we found that the 1 can selectively detect the hNQO1 activity with a high specificity constant (kcat/Km = 5.393 ± 1.531 X 106 M-1min-1), which was relatively higher value compared to other probe reported earlier. |
| E-mail |
naome0609@sookmyung.ac.kr |
|
121st General Meeting of the KCS