121st General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ORGN.P-362
Subject A noble two-photon excitable far-visible FRET system for imaging mitochondrial pH
Authors Min Jung Chang, Chulhun Kang1,*, Min Hee Lee2,*
Department of chemistry, Sookmyung Women's University, Korea
1Graduate School of East-West Medical Science, Kyung Hee University, Korea
2Department of Chemistry, Sookmyung Women's University, Korea
Abstract

We developed a two-photon excitable mitochondria-selective pH probe (1) composed of naphthalimide and rhodamine dyes linked to a piperazine. In response to a pH variation, the 1 gave a ratiometric fluorescence change at 598 nm based on a combination of PET and FRET mechanisms. This change was reversible and rapidly detectable without interfering by other biological species. In addition, the 1 was found to be localized to the mitochondria of live HeLa cells and it gave a ratiometric fluorescence enhancement upon the acidification of mitochondria using CCCP treatment and nutrient starvation conditions.

E-mail minminkong@sookmyung.ac.kr