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Type |
Poster Presentation |
Area |
Organic Chemistry |
Room No. |
Event Hall |
Time |
4월 19일 (목요일) 11:00~12:30 |
Code |
ORGN.P-362 |
Subject |
A noble two-photon excitable far-visible FRET system for imaging mitochondrial pH
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Authors |
Min Jung Chang, Chulhun Kang1,*, Min Hee Lee2,* Department of chemistry, Sookmyung Women's University, Korea 1Graduate School of East-West Medical Science, Kyung Hee University, Korea 2Department of Chemistry, Sookmyung Women's University, Korea |
Abstract |
We developed a two-photon excitable mitochondria-selective pH probe (1) composed of naphthalimide and rhodamine dyes linked to a piperazine. In response to a pH variation, the 1 gave a ratiometric fluorescence change at 598 nm based on a combination of PET and FRET mechanisms. This change was reversible and rapidly detectable without interfering by other biological species. In addition, the 1 was found to be localized to the mitochondria of live HeLa cells and it gave a ratiometric fluorescence enhancement upon the acidification of mitochondria using CCCP treatment and nutrient starvation conditions. |
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E-mail |
minminkong@sookmyung.ac.kr |
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