121st General Meeting of the KCS

Type Poster Presentation
Area Material Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code MAT.P-359
Subject Surface charge effect of graphene oxides in blood
Authors Hyoung-Mi Kim, Jae-Min OH*
Chemistry and Department of Medical Chemistry, Yonsei University, Korea
Abstract Graphene oxide (GO) is attracting nanomaterials due to potential applications in various application fields. We prepared GO to have controlled surface chemistry by modifying with amine moieties such as ammonia, 1,3-diaminopropane and 1,6-diaminohexane, respectively. The X-ray diffraction pattern showed that structure of GO and modified amine moieties. X-ray photoelectron spectroscopy results exhibited that the successful formation of C-O and C-N bonds in amine modified GOs (GNs). From zeta potential analysis, GO showed strong negative charge at all the measured pH and showed that the zeta potential changed from negative (~ -45 mV) to positive (~+33 mV) values upon amine modification with increasing alkyl chain of amine moieties. In order to evaluate surface charge effect of GO on blood cell, we carried out hemolysis and cell counting assay utilizing human whole blood. The surface interaction between GOs and blood cells was observed either by optical microscopy after Wright staining or by scanning electron microscopy. To evaluate interaction between proteins and GOs depending on their surface chemistry, we investigated both protein adsorption isotherms by Bradford assay and protein denaturation/aggregation by fluorescence quenching assay utilizing human serum albumin, human serum gamma-globulin, human serum fibrinogen and human whole plasma, respectively. The results revealed that the samples have more interaction with increasing negative surface charge.
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