|
Type |
Symposium |
Area |
Molecular Bioimaging and Cutting Edge Techniques |
Room No. |
Room 203 |
Time |
THU 16:00-: |
Code |
BIO1-2 |
Subject |
Go hybrid : Single particle EM and X-ray crystallography for macromolecular protein complex |
Authors |
Ho Min Kim Graduate School of Medical Science & Engineering, Korea Advanced Institute of Science and Technology, Korea |
Abstract |
Transmission Electron Microscopy (TEM) has long been utilized in the various research fields including material science, nanotechnology and semiconductor research as well as biology and medical science. Recently, single-particle EM has emerged as a mainstream structural biological technique, enabling structure determination of large protein complexes to atomic or near-atomic resolution. The current success of single-particle EM has been led by advances in microscope design, cryogenic sample preparation, image processing software, fast and high sensitive detector as well as automatic data acquisition. However, some protein complexes are intrinsically difficult to reach the high-resolution by single-particle cryoEM. Therefore, in those cases, a hybrid approach (X-ray crystallography, single-particle EM and Single Molecule Fluorescence analysis etc) is the only way to go. Here, I will introduce several examples of hybrid approaches that can reveal the molecular mechanism of dynamic protein interactions (Lipopolysaccharide transfer mechanism for innate immune response and synaptic adhesion complex for synaptogenesis) as well as a high-resolution cryoEM structure that was utilized to design protein scaffolds for drug delivery. |
E-mail |
hm_kim@kaist.ac.kr |
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