121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-264
Subject Electrical sensor based on aptamer for distinguishing between Influenza virus H1 and H5
Authors Yong-Joo Jeong
Department of Applied Chemistry, Kookmin University, Korea
Abstract The surface protein hemagglutinin (H) plays an important role in the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. In this study, we described a method for the selective electrical detection of subtype H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype. Measurements by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide (ITO)-coated surface was specifically bound to the H1 subtype but not the H5 subtype, which was consistent with ELISA and FE-SEM. Further measurement of CV using various concentrations of H1 subtype provided detection limit of the immobilized aptamer, which showed that nanomolar scale of target protein was sufficient to produce the signal.
E-mail jeongyj@kookmin.ac.kr