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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-237 |
| Subject |
Efficient Enrichment of Phosphopeptides on Digital Microfluidics (DMF) Chip Using TiO2-Magnetic Bead by MALDI-TOF MS |
| Authors |
jinwoo kim, In-Ae Jang, Han Bin Oh*
Department of Chemistry, Sogang University, Korea
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| Abstract |
Phosphorylation is one of the most important post-translational modifications (PTMs) of proteins, which modulates a wide range of biological functions and activity of proteins. The analysis of phosphopeptides is still one of the most challenging tasks in proteomics research by mass spectrometry. In this study, phosphopeptide enrichment approach on a digital microfluidic (DMF) chip was demonstrated by analyzing phosphopeptides in the tryptic digested β-casein (bovine) and ovalbumin (chicken). This approach was made using a magnetic bead (MB)-based titanium dioxide (TiO2)-solid phase extraction(SPE) procedure. TiO2-MB was employed to selectively enrich phosphopeptides from tryptic digests of β-casein and ovalbumin. The enriched phosphopeptides were detected using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This study shows that the phosphopeptide enrichment analysis can be automated and analyzed with a small sample volume on the DMF chip. |
| E-mail |
mylooda@naver.com |
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121st General Meeting of the KCS