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|
| Type |
Poster Presentation |
| Area |
Material Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
MAT.P-377 |
| Subject |
Modulation of mammalian cell-cell interaction using zwitterionic lipid masks |
| Authors |
Wongu Youn, INSUNG CHOI*
Department of Chemistry, Korea Advanced Institute of Science and Technology, Korea
|
| Abstract |
Cells in vivo face a complex microenvironment, not only composed of a diverse array of biomolecular stimulants and structural cues, but also several heterotypic cell populations. As a result, the study of cell-cell interactions has become especially important. However, conventional methods for in vitro cell culture are ill-suited to modulate cell-cell interactions that occur. In order to improve the degree of control over cell-cell interactions, several ‘patterned co-culture’ platforms have been proposed. But those methods have some drawbacks, ranging from biocompatibility, pattern shape restriction, complicated methods, and demanding severely restrictive materials and conditions. We propose a simple and highly biocompatible means of generating patterned co-cultures through the use of lipids, a common biomolecule, as an easily removable, cell-repellant mask. Lipids, depending on their charge, can possess varying degrees of cell-repellence. Also, they are easily removable. So, we created a ‘lipid-mask’-based platform to form patterned co-cultures of heterotypic cell lines. Zwitterionic lipid masks selectively blocked cell adhesion to the substrate during the initial seeding, and limited cell attachment to microcontact-printed protein barriers. The masks were then removed via a routine washing step under physiological conditions, exposing the underlying substrate for protein coating and secondary cell seeding. The lipid-mask-based platform allowed for an extremely facile and rapid means of creating patterned co-cultures under highly biocompatible conditions. |
| E-mail |
togosori@kaist.ac.kr |
|
121st General Meeting of the KCS