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| Type |
Poster Presentation |
| Area |
Inorganic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
INOR.P-82 |
| Subject |
Mitochondria Labeling for Magnetic Bioimaging with Nanodiamond Bioconjugates |
| Authors |
Yoobeen Lee, Yi-Seul Park, JIN SEOK LEE*
Department of Chemistry, Sookmyung Women's University, Korea
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| Abstract |
Nanodiamonds (NDs) have excited a lot of interest in the field of bioimaging as they can be used as spin probes for electromagnetic sensing of cellular and biological environments. While a variety of surface functionalizations have enabled endosomal uptake of the NDs or targeting to the cell surface, very few studies have investigated selective targeting of ND conjugates to organelles and subcellular compartments within living cells. This lack is due in large part to the limited number of intracellular delivery options, which has constrained the ND surface chemistry to functional groups which facilitate cellular uptake. As a result, since intracellular targeting groups have no mechanism by which the functionalized NDs can penetrate the cell membrane, intracellular targeting has yet to be conclusively addressed with multiple sites targeted and fluorescence colocalization experiments to confirm proper targeting.
In this study, the ND bioconjugates were delivered into mitochondria in human skin cells (WS1) for subsequent conjugation reactions. Also, we set up the confocal diamond microscope for detection and imaging of labeled cells. The instrument checks nitrogen-vacancy (NV) centers in NDs for correlated magnetic and fluorescence imaging. Fluorescence colocalization experiments confirmed specific targeting of the ND bioconjugates to mitochondria. This directed subcellular targeting could enable spatiotemporal resolved measurements of ionic flow or local changes in concentration of reactive oxygen species within living cells. |
| E-mail |
yoobeen@sookmyung.ac.kr |
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121st General Meeting of the KCS