121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-266
Subject Neuro-Compatible Metabolic Labeling of Sialoglycan of Primary Hippocampal Neurons in Noncontact Neuron–Astrocyte Coculture
Authors JI YU CHOI, KYUNGTAE KANG1,*, INSUNG CHOI*
Department of Chemistry, Korea Advanced Institute of Science and Technology, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
Abstract Glycans are important in several facets of neuronal development and disease. However, the metabolic labeling of surface sialoglycans in primary neurons is a difficult task because of the neurotoxicity of unnatural monosaccharides that are used as a metabolic precursor, which disturbing the progress of metabolic engineering in nerve fields. Therefore, in this paper, we report a neuro-compatible, sandwich neuron–astrocyte coculture system that neutralizes the neuro-toxicity of unnatural monosaccharides, allowing for the long-term tracking and characterization of sialoglycans in primary neurons in vitro, for up to 21 DIV. Two-color labeling via the metabolic labeling with peracetylated N-azidoacetyl-d-mannosamine (Ac4ManNAz), shows that neuronal activities, such as neurite outgrowth and recycling of membrane components, are highly dynamic and change over time during development. Especially in neurite elongation, the insertion sites of membrane components are suggested to not be random, but be predominantly localized in developing neurites. This work provides a research platform for metabolic-labeling studies of glycans in primary neurons.
E-mail bbchoi73@kaist.ac.kr