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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-595 |
| Subject |
A new radiolabeling technique using SpyCatcher/SpyTag tool |
| Authors |
Jung-Min Jung, Phoung Tu Huynh, Yeong Su Ha, JUNG-YOUNG KIM1, Kyo-Chul Lee1, Mark Howarth2, Jeongsoo Yoo*
Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University, Korea
1RI-Convergence Research, Korea Institute of Radiological & Medical Science, Korea
2Biochemistry, University of Oxford, United Kingdom
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| Abstract |
Conventional radiolabeling for antibodies is a two step process where first chelator-antibody bioconjugates are formed, and then followed by radiolabeling. Thus, labeling site is randomized, conjugation yield is low and the bioactivity may also be compromised. New technique of site-specific labeling of radioisotope utilizing enzyme-mediated SpyCatcher/SpyTag was developed in this study and its usefulness was tested on radiolabeling of biololecules. Here, we have developed a novel probe (64Cu-SpyTag-SpyCatcher-AffiHer2) for targeting human epidermal growth factor receptor type 2 (HER2). This probe can differentiate between HER2 over-expressed cells and HER2 deficient cells in in vitro studies. In the ex vivo biodistribution studies, the tumor targeting by 64Cu-SpyTag-SpyCatcher-AffiHer2 was confirmed in xenograft tumor models. These results shows that this site-specific radiolabeling strategy for biomolecules is highly specific, efficient and convenient, with no attenuation of the targeting ability. |
| E-mail |
jjm1991xn@hanmail.net |
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121st General Meeting of the KCS