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Type |
Poster Presentation |
Area |
Physical Chemistry |
Room No. |
Event Hall |
Time |
4월 20일 (금요일) 11:00~12:30 |
Code |
PHYS.P-111 |
Subject |
Rebinding Dynamics of Carbon monoxide with Cytoglobin using Time-resolved Infrared Spectroscopy |
Authors |
Manho Lim*, JuHyang Shin Department of Chemistry, Pusan National University, Korea |
Abstract |
Femtosecond and nanosecond vibrational spectroscopy was used to probe the rebinding dynamics of CO to cytoglobin (Cgb) after photodeligation of the corresponding CO-bound protein in D2O buffer (pD = 7.4) at 273 K. When excited with a 532-nm photon, carbon monoxide dissociated immediately from cytoglobin (< 0.2 ps), ground state bleaching of CO bound to the cytoglobin has three conformations (A0, A1 and A3). Each conformation shows characteristic 13C−O stretching frequencies. The vibrational spectrum of CO in Cgb13CO described three gaussian at 1924.6 cm-1 (A0), 1900.2 cm-1 (A1) and 1884.5 cm-1 (A3) with a full width at half-maximum (FWHM) of 16 cm-1, 15.8 cm-1 and 12.5 cm-1. Ligand binding to Cgb is a multiphasic process with fast geminate rebinding taking place on the nanosecond timescales and slower biomolecular rebinding occurring on microsecond timescales.
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E-mail |
dksvmflzk@gmail.com |
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