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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-247 |
| Subject |
Identification of novel disulfide adducts containing leaving group of the nerve agents VX from human serum albumin by LC-MS/MS |
| Authors |
JinYoung Lee*, Yong Han Lee
Agency for Defense Development, Korea
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| Abstract |
Verification of exposure to Chemical Warfare Agents (CWAs) plays an important role in the way that unambiguous confirmation of an alleged use of banned substances by terrorist forces may have reaching political and legal impact. However, direct detection of CWA in vivo is quite difficult due to rapid clearance from the circulation and structural changes in the course of biotransformation processes. Current efforts are focused on identification of adducts between CWA and endogenous target proteins, as these biomarkers are still detectable days or even weeks after exposure.
Human serum albumin(HSA) is one of target protein which was inhibited by VX (O-Ethyl S-2-diisopropylaminoethyl methyl phosphonothiolate) on Tyrosine 411. Consideration of covalent protein modification by VX typically refers to phsphylation of nucleophilic amino acid residues. In contrast, the result on covalent modification by the respective leaving groups of 2-diisopropylaminoethanethiol(DPAET) are lacking in the literature. The aim of the present study was to examine reactions of the nerve agent VX forming the already known O-ethyl methyl phosphonlyated tyrosine residues as well as potential novel disulfide adducts between its thiol containing leaving group DPAET and HSA. We newly developed the sample preparation method for detecting two biomarkers from the VX inhibited human plasma simultaneously.
Keywords: albumin, disulfide adduct, chemical warfare agent, LC-MS/MS |
| E-mail |
marlintime@add.re.kr |
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121st General Meeting of the KCS