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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-273 |
| Subject |
Raman-Based In-situ Monitoring for the Changes of Molecular Signatures During Plasmonic photo-thermal induced Apoptosis
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| Authors |
HyeonJeong Shin, DONGKWON LIM*
KU-KIST Graduate School, Korea University, Korea
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| Abstract |
From a molecular biological point of view, methods for analyzing apoptosis include annexin V & PI staining, identifying proteins expressed in the cell death process and determining cell death through live Cell imaging. But these biological analysis-based methods are not possible in the process of cell death in real time but only confirm the cell death results. Raman technology, which is used for sensing and imaging in the biomedical field, is a suitable technique to detect changes in molecular units in a time-dependent manner. It can detect the changes of specific substance in the states where apoptosis has been induced by detecting the Raman signal. Under the conditions in which apoptosis was induced at the cellular level, it is considered to be an effective tool for monitoring cell changes. The gold nanoparticles have been tremendously used in biomedical application due to their ease of colloidal synthetic strategies, control over their size and shape, facile surface modification and also has high biocompatibility. It can also be used in SERS due to the unique optical properties of LSPR. Here, we observe the Raman signal change by identifying the apoptosis over time by inducing plasmonic photothermal damage to cells using gold nanoparticle actively targeted to specific cell organelles.
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| E-mail |
friend558@korea.ac.kr |
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121st General Meeting of the KCS