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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-274 |
| Subject |
Enrichment of Single-stranded DNA Aptamers for Detecting H3-HA1 from H1-HA1 and Yam-HA1 |
| Authors |
Tae Gyun Lee, Yong-Joo Jeong*
Department of Applied Chemistry, Kookmin University, Korea
|
| Abstract |
Influenza viruses that get worse in winter are a pandemic respiratory disease with a wide variety of subtypes. It is necessary to detect each influenza virus subtype from the specimen for target-dependent treatment such as vaccination against each influenza virus. In this study, we isolated DNA aptamer to detect hemagglutinin, which is a characteristic of influenza virus, especially HA1 domain, which plays a major role in influenza infection. We have identified an aptamer that can distinguish H3N2 (H3), H1N1 (H1), and Yamagata (Yam) through the Counter-SELEX method and identified an aptamer that binds only to H3-HA1. After a total of 15 rounds of counter-SELEX, we found that the aptamer binds to only H3-HA1 but not H1-HA1 and Yam-HA1 by conducting ELISA. With this aptamer, we can be utilized as a diagnostic kit that detects H3N2. |
| E-mail |
haroun90@naver.com |
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121st General Meeting of the KCS