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| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ORGN.P-416 |
| Subject |
A Cy7-Based Ratiometric Fluorescent Probe Targeting for Mitochondrial Nitroreductase |
| Authors |
WON JOO LEE, seokan Park, Hae-Jo Kim*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
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| Abstract |
Background: Hypoxia can cause an increase in reductive stress so nitroreductase (NTR) can be overexpressed in hypoxic tumors. Therefore, the detection of hypoxia and NTR is a feasible tool for the diagnosis of tumor cells and warrants robust detection for a biological study.1,2
Materials and Method: Chemical probes were prepared according to the standard method in the lab and NTR enzyme together with NADH was purchased from the chemical suppliers.
Result: A heptamethinylcyanine (Cy7) based dual optical probes were developed for detection of nitroreductase (NTR). Upon addition of NTR in the presence of NADH, the probe exhibited dramatic optical changes with strong fluorescence (F/F0 = 3.4, λem 747 nm) as well as intense blue color (λabs 620 nm) by the nitro-to-amine functional group transformation and displayed a limit of detection (LOD = 36.9 ng/mL) of NTR in PBS buffer. Further application to endogenous cellular NTR was successfully applied in HeLa cells, showing that the probe was clearly localized in a mitochondrial region.
Conclusion: Latent chromogenic and fluorogenic probe was successfully applied for the detection of mitochondrial NTR in vivo as well as in vitro.
References
1. Xu, K.; Wang, F.; Pan, X.; Liu, R.; Ma, J.; Kong, F.; Tang, B. Chem. Commun., 2013, 49, 2554.
2. Li, Y.; Sun, Y.; Li, J.; Su, Q.; Yuan, W.; Dai, Y.; Han, C.; Wang, Q.; Feng, W.; Li, F. J. Am. Chem. Soc., 2015, 137, 6407
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| E-mail |
wonjoo721@gmail.com |
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121st General Meeting of the KCS