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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-255 |
| Subject |
Detection of tryptic peptide of ricin according to the concentration of maltodextrin using LC-MS/MS |
| Authors |
JIHYUN KWON*, Yong Han Lee
Agency for Defense Development, Korea
|
| Abstract |
Ricin is a toxic protein derived from castor beans and consist of a cytotoxic A chain and B chain covalently linked by a disulfide bond, which can inhibit protein synthesis and cause cell death by binding the surface of cell. Ricin is considered a likely agent for bioterrorism because of its high toxicity. If purified into a terrorist or warfare agent, ricin could be used to expose people through the air, food or water via injection. Therefore sensitive and selective analytical methods are needed to detect ricin in complex food matrices. In this work, we used the maltodextrin, which is artificial sweetener in a variety of other processed foods, as a matrix and MB-Ab(Magnetic beads-Antibody) was used to capture the ricin in maltodextrin. Tryptic peptides of ricin were analyzed to determine if the concentration of maltodextrin affected the ricin capture of MB-Ab by liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
| E-mail |
jhkwon8@add.re.kr |
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121st General Meeting of the KCS