|
|
| Type |
Poster Presentation |
| Area |
Inorganic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
INOR.P-110 |
| Subject |
Multicolor Quantum Dot Labeling of Both of Hydrophilic and Hydrophobic Regions of Liposome
|
| Authors |
Yeonki Hong, Daeha Seo*
Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science & Technology, Korea
|
| Abstract |
It is now recognized that plasma membrane regulating all protein functions by generating a variety of micro-
domains. Despite importance of plasma membrane in spatiotemporal dynamics, direct imaging probe to observe
the lipid bilayer in live cell is limited compared with other membrane receptors and their compartments, because
the imaging probe should overcome the chemical potential between hydrophilic and hydrophobic layers. Here,
we constructed probe delivery systems to the plasma membrane by generating hybrid nanostructures composed
of liposome and inorganic nanocrystals, quantum dots (QDs). Since the thickness of lipid bilayer is about 4 nm,
QDs are suitable optical probe which is typically between 2 and 10 nm in size. We prepared small unilamellar
vesicles (SUVs) composed of trioctylphosphine oxide-coated green QDs embedded in lipid bilayer and
mercaptopropionic acid-coated red QDs encapsulated in SUV. Fluorescence microscopy images of the SUVs
show a colocalization of hydrophobic and hydrophilic QDs. Such system should find broad utility in studies
requiring single-molecule imaging either in cytosolic or in lipid membrane. |
| E-mail |
hongyeongy@naver.com |
|
121st General Meeting of the KCS