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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-256 |
| Subject |
Direct Detection of Influenza A Virus DNA based on Scattering Signal of Plasmon Metal without Amplification |
| Authors |
Seungah Lee, Seong Ho Kang*
Department of Applied Chemistry, Kyung Hee University, Korea
|
| Abstract |
The direct detection of influenza virus DNA without amplification effectively was performed by scattering signal of novel nanometals through grating. Gold nanopad and silver nanometal were used as a substrate for immobilization of probe single-stranded DNA (ssDNA) and as detection tag. The grating separated the scattering signals of the nanotag from that of the substrate and removed strong background noise or nearby interference. This sandwich hybridization-based biosensor quantified the target molecule until zeptomolar (zM, 10-21 M) concentration and a dynamic range of six orders of magnitude by grating associated total internal reflection scattering (TIRS) microscopy. The relative scattering intensity of blank controls (without H7N9 and H5N1 virus DNA) was 6 times lower than that of the H7N9 virus DNA. The results showed that this biosensor could specifically recognize the target DNA fragment of influenza A (H7N9) virus from other types of influenza viruses. |
| E-mail |
moon1131@gmail.com |
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121st General Meeting of the KCS