121st General Meeting of the KCS

Type Poster Presentation
Area Electrochemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code ELEC.P-587
Subject DT-Diaphorase as a Bifunctional Enzyme Label for High Signal Amplification
Authors Junguk Kwon, Haesik Yang*
Department of Chemistry, Pusan National University, Korea
Abstract The most common enzyme labels in enzyme-linked immunosorbent assays are alkaline phosphatase and horseradish peroxidase, which, however, have some limitations for use in electrochemical immunosensors. We report that the small and thermostable DT-diaphorase (DT-D) and electrochemically inactive 4-nitroso-1-naphthol can be used as a bifunctional enzyme label and a rapidly reacting substrate, respectively, for electrochemical immunosensors. This enzyme−substrate combination allows high signal amplification via rapid enzymatic amplification and electrochemical redox cycling. DT-D can convert an electrochemically inactive nitroso or nitro compound into an electrochemically active amine compound, which can then be involved in electrochemical−chemical (EC) and electrochemical−enzymatic (EN) redox cycling. The electrochemical immunosensor using DT-D and 4-NO-1-N detects parathyroid hormone (PTH) in phosphate-buffered saline containing bovine serum albumin over a wide range of concentrations with a low detection limit of 2 pg/mL. When the PTH concentration in clinical serum samples is measured using the developed immunosensor, the calculated concentrations are in good agreement with the concentrations obtained using a commercial instrument. Thus, the use of DT-D as an enzyme label is highly promising for sensitive electrochemical detection and point-of-care testing.
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