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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-609 |
| Subject |
Development of a BODIPY-based fluorescent sensor for imaging tau aggregates in live cells |
| Authors |
Sungsu Lim, Yun Kyung Kim*
Korea Institute of Science and Technology, Korea
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| Abstract |
Accumulation of tau aggregates in neurons is a pathological hallmark in multiple neurodegenerative disorders, collectively called tauopathies. To facilitate the study of tau aggregation processes and the discovery of tau aggregation blockers, tau aggreagtion sensor that can monitor abnomal tau aggregation in neurons is important. Here, we describe that a BODIPY-fluorescence sensor (BD-tau) selectively responds pathological tau aggregates in live cells. We developed BD-tau, a BODIPY-based fluorescent sensor that detects pathological tau aggregates live cells. First, BD-tau is a fluorescence turn-on sensor. The quantum yield of BD-tau increased 8-fold upon binding to tau aggregates in vitro. β-sheet protein panel assay indicates that BD-tau has a superior specifically to tau aggregates over amyloid plaques or insulin aggregates. Second, BD-tau is a cell-permeable imaging probe. In live hippocampal neurons, BD-tau selectively labelled pathological tau aggregates. The live-cell imaging property of BD-tau might be aided by high cell-permeability property of BODIPY scaffold. In addition to that, BD-tau, as a live-cell imaging probe for detecting tau aggregates, will facilitate the investigation of tau pathology and the discovery of anti tau-aggregates drugs. |
| E-mail |
sungsulim85@gmail.com |
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121st General Meeting of the KCS