121st General Meeting of the KCS

Type Poster Presentation
Area Inorganic Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code INOR.P-117
Subject Chemical Toolkit for Spatiotemporal-Quantitative Manipulation of Intracellular Signaling
Authors Sangwon Shin, Jaeheung Cho1,*, Daeha Seo*
Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science & Technology, Korea
1Emerging Materials Science, Daegu Gyeongbuk Institute of Science & Technology, Korea
Abstract Systems biology is a collective understanding of the composition, relationships, interactions, and dynamics of the components that make up the system in which biological phenomena occur, in order to uncover the fundamental principles governing complex life phenomena. System biology requires tools that can regulate the activity of molecules and proteins in the cell, both spatiotemporally and quantitatively, as well as biological sensors that can measure the output of cell signals. We synthesized inorganic compounds that have photolytic ligands to activate target cell signal and constructed biological sensors through plasmid cloning and transfection. The cell permeable cobalt complex [CoⅢ(MDAP)(CH3CN)(NO)](ClO4)2 releases NO by receiving light in the cell, which activates the ERK signal in the cell. By activating the ERK signal of the biological sensor model cell, the change of the cell signal can be observed in real time by fluorescence microscopy. It is based on the study of system biology by enabling spatiotemporal and quantitative regulation of cells and observing changes in cellular signals.
E-mail sang131212@gmail.com