121st General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ANAL.P-257
Subject A simple method for identification of caffeic and rosmarinic acid in Thymus vulgaris by using HPLC
Authors XINGFU YIN, In Hye Kim, Seungui Cheon, Seungah Lee1, Jaehun Jeong1, Yoohun Noh*
Research dept., Famenity, Korea
1Research dept., Naturesense, Korea
Abstract Rapid identification and quantification of plant components using chromatographic techniques such as ultraviolet spectroscopy (UV), high-performance liquid chromatography (HPLC) and mass spectroscopy (MS) are widely employed for investigating crude extracts. In this study, evaluation of a 50% ethanolic extract from Thymus vulgaris (T.vulgaris) was performed using modern chromatographic techniques to determine the main extract components. The present study describes the characterization of three compounds in extracts from T.vulgaris from Turkey using HPLC and UV analysis. We previously showed that the major compound in T. vulgaris is rosmarinic acid. In the present study, we qualitatively and quantitatively analyzed rosmarinic acid and caffeic acid in T. vulgaris ethanolic extracts. HPLC revealed a third peak with the same retention time as pectolinarin, but LC/MS indicated that it was another compound. The obtained results could prove valuable for standardization of T. vulgaris compounds. Our findings could be used as a basis for clinical research and chemical profiling of thyme and other plant.
E-mail yxf8523@hanmail.net