121st General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Event Hall
Time 4월 19일 (목요일) 11:00~12:30
Code ANAL.P-258
Subject A glucose dehydrogenase enzyme based glucose sensor via ruthenium polymer redox complex
Authors Chang Jun Lee, Soo Yeon Jeon, Won-Yong Jeon, Young Bong Choi, Hyug-Han Kim*
Department of Chemistry, Dankook University, Korea
Abstract Modified electrodes that react with glucose dehydrogenase (GDH) using water-soluble hydrogel mediator were fabricated for glucose assay. Glucose electrodes based on hydrogels were prepared by crosslinking GDH and the redox polymer formed with PVP-PAA-[Ru(ter-py)]+/2+ (ter-py = 2,2’-6’,2’’-therpyridine) on screen printed carbon electrodes (SPCEs) surface. The PVP-PAA-[Ru(ter-py)]+/2+redox mediator was characterized by NMR, UV-vis, and XPS spectroscopy, and electrochemically by CV. The Electrochemical Impedance Spectroscopy (EIS) was used to investigate surface changes of modified electrodes. The difference of the current value according to presence or absence of oxygen was examined through CV. Also, i-t curves about different glucose level showed linearly. The effect of interfering substances such as ascorbic acid, dopamine, and uric acid was examined through the i-t curve. Therefore, this ruthenium complex can be used for glucose detection with GDH as a good substitute mediator.
E-mail chang5654@naver.com