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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Event Hall |
| Time |
4월 20일 (금요일) 11:00~12:30 |
| Code |
BIO.P-279 |
| Subject |
3D bioprinting of bifurcating vessels for hierarchical vasculature |
| Authors |
Giho Choe, Kwanwoo Shin*, Kihoon Kim, Minyoung Kim1
Department of Chemistry, Sogang University, Korea
1Chemistry, Sogang University, Korea
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| Abstract |
Despite the significant advancements in tissue engineering to mimic native tissue constructs, fabrication of bifurcating vessels and vascularized tissue constructs still pose a great challenge. The importance of vascular networks in tissue constructs is that vascularization provides nutrients with and remove cellular waste from the cells in tissue. Here, we present a novel method to bioprint bifurcating vessels and develop hierarchical vasculature. In particular, we designed a diffusive bioprinting method by incorporating calcium chloride in Pluronic F127 (PFCa) and casting of alginate/gelatin mixture over the extruded PFCa ink. Pluronic F127 as well as blend with calcium chloride shows Bingham plastic properties which enable preservation of extruded filamentary shape and bifurcation of the vessels. After casting alginate/gelatin mixture on extruded PFCa, calcium circumferentially diffuse and crosslink alginate, yielding a tubular hydrogel construct that physically mimics vessels. The structural properties of the vessels are tuned by changing the calcium diffusion time and the concentration of calcium in PFCa. This diffusive bioprinting technique enables bifurcation of the vessels and hierarchical vasculature that obey Murray’s law, such that the cost for transport and maintenance of the transport materials is minimized. The bifurcating vessels and hierarchical vasculature could improve the material flow between cells which in turn promote cell viability over time in artificial tissue constructs.
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| E-mail |
gihochoi@sogang.ac.kr |
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121st General Meeting of the KCS