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| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
ORGN.P-447 |
| Subject |
Development of an enolase detection assay for the treatment of rheumatoid arthritis |
| Authors |
YeongMok Kim, Sang Jeon Chung1,*
Pharmarcy, Sungkyunkwan University, Korea
1Department of Chemistry, SungKyunKwan University, Korea
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| Abstract |
Enolase, a metalloenzyme, plays a crucial role in the human body and is over expressed in more than 95% of immune cells of rheumatoid arthritis (RA) patients. Abnormal quantities of enolase are known to activate the immune system leading to an increase of inflammatory mediators such as TNF-α and interleukin, the main triggers of arthritis. Immunosuppressive drugs such as nonsteroidal, steroids, and TNF blockers can be used to alleviate RA. However, presently there is no cure for this disease. To facilitate the discovery of new therapeutic agents to treat RA, an enolase detection assay system to screen potential enolase inhibitors is necessary. Here, we report the development of a fluorescence based enolase detection assay using an active site specific peptide conjugated to a fluorophore. Our developed assay employs enolase immobilized on a chip and allows the high throughput screening of large libraries of potential enolase inhibitors. Potent and selective enolase inhibitors may pave the way for new therapeutic treatments of RA. |
| E-mail |
rusynante@naver.com |
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121st General Meeting of the KCS