121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-280
Subject Highly Sensitive Near-Infrared Fluorescence Probe for Nitroreductase Imaging
Authors Sun Hyeok Lee, Kwangmo Go1, Chul Soon Park2, Chang-Soo Lee3,*
Department of Bio Engineering, University of Science & Technology, Korea
1Department of Chemical Engineering and Applied, Chungnam National University, Korea
2Polymer Engineering, Chonnam National University, Korea
3Center for Bio Nano Research, Korea Research Institute of Bioscience and Biotechnology, Korea
Abstract Nitroreductase (NTR) is a protein which reduces nitrogen-containing compounds to amine group. Especially, NTR can be overexpressed in tumor cells under hypoxia condition, therefore, it is greatly important to detect NTR for diagnosing affected parts where tumor cells grow. Up to date, the effective methods for imaging NTR are still lacking even if a lot of approach has been reported to detect NTR in solution. Thus, it is required to develop new probes for intracellular or animal NTR imaging for a long time without photobleaching. In this study, we designed and synthesized novel near-infrared (NIR) probes, which have a hemicyanine skeleton (HXPI) moiety, to detect NTR in aqueous media. Fluorescence of the NIR probes was enhanced when reduced from nitro group to amine group by NTR. We confirmed that NTR expression level was increased in tumor cells under hypoxia condition though NTR imaging.
E-mail tnsgur02@kribb.re.kr