121st General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Event Hall
Time 4월 20일 (금요일) 11:00~12:30
Code BIO.P-281
Subject Structural investigation of riboswitch folding important for regulation of bacterial gene expression
Authors Ji-Yeon Shin, Kyeong-Mi Bang, Nak-Kyoon Kim1,*
Korea Institute of Science and Technology, Korea
1Advanced Analysis Center, Korea Institute of Science and Technology, Korea
Abstract Riboswitch is a structural RNA motif that is located at the 5’- end of mRNA, regulates protein expression upon binding small molecules. Riboswitches are composed of the aptamer domain and the expression platform. The aptamer domain specifically binds a ligand and the expression platform is responsible for the gene expression through its ability to switch the conformation between two different secondary structures in response to ligand binding. We have studied the cyclic-di-GMP (bis-(3′-5′)-cyclic dimeric guanosine monophosphate,) riboswitch. Cyclic-di-GMP is widely used by bacteria to regulate processes ranging from biofilm formation to the expression of virulence genes. Cyclic-di-GMP riboswitch is the first known example of a gene-regulatory RNA that binds a second messenger, and it is present in pathogens such as Clostridium difficile, Vibrio cholerae and Bacillus anthracis. We investigated the structural mechanism of ligand binding of c-di-GMP riboswitch using NMR spectroscopy. The proton and carbon resonances of the riboswitch were assigned, and the secondary structure was determined by NMR. We also monitored the conformational changes of riboswitch RNA upon Addition of Mg2+ and c-di-GMP to the riboswitch. Investigation the tertiary structure and ligand recognition mechanism of the riboswitch is essential to understand the gene regulation mechanism of the riboswitch. Our results will provide an insight into the new design of RNA targeting antibiotics.
E-mail 215507@kist.re.kr