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| Type |
Poster Presentation |
| Area |
Medicinal Chemistry |
| Room No. |
Event Hall |
| Time |
4월 19일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-620 |
| Subject |
Anti-oxidative compounds from Galla Rhois |
| Authors |
Heesu Kwon*, gyusun jeong, Bong Ho Lee1,*, Byong Wook Choi*
Department of Chemical & Biological Engineering, Hanbat National University, Korea
1Division of Applied Chemistry & Biological Enginee, Hanbat National University, Korea
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| Abstract |
In our continuing search for anti-oxidative, anti-cholinesterase and anti-tyrosinase compounds from oriental medicinal plants, Galla Rhois was examined. Here we report the progress on the purification, biological activities and structural determination of the two isolated compounds.
From the 100% methanol extract of Galla Rhois, solvent partition fractions were prepared by using n-hexane(14-GRM-H), chloroform(14-GRM-C), butanol(14-GRM-B) and water(14-GRM-W). Among them 14-GRM-B showed strong anti-oxidative, *BuChE inhibitory and tyrosinase inhibitory activities. The 14-GRM-B of Galla Rhois showed 84% DPPH radical scavenging activity, 81% BuChE inhibitory activity and 62% tyrosinase inhibitory activity at 100 ppm. Using a series of ODS open column and Sephadex LH-20 column chromatographic purification steps, two compounds, 14-GRM-B-1-3 and 14-GRM-B-1472 could be purified. At 100 ppm, they showed anti-oxidative activity as 92.0, 74.8%, respectively. The chemical structure of 14-GRM-B-1-3 was determined by 1H NMR and comparison of HPLC chromatogram with reference compounds. The chemical structure of 14-GRM-B-1472 was determined by 1H, 13C NMR, 2D NMR(HSQC and COSY NMR) and MS analysis. 14-GRM-B-1-3 and 14-GRM-B-1472 were turned out to be gallic acid and trigalloylglucose(TGG), respectively.
* BuChE : Butyrylcholinesterase |
| E-mail |
gmltn5512@naver.com |
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121st General Meeting of the KCS